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KMID : 0357820040280020006
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Korean Journal of Legal Medicine
2004 Volume.28 No. 2 p.6 ~ p.13
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Intra-individual Difference of Length Heteroplasmy in Blood and Hair Shaft Mitochondrial DNA
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Chung Uk-Hee
Lee Hwan-Young
Park Myung-Jin
Yoo Ji-Eun
Han Gil-Ro
Cho Sang-Ho
Kim Chong-Youl
Shin Kyoung-Jin
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Abstract
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To observe mtDNA length heteroplasmy in a homoploymeric cytosine tract of the mitochondrial HV2 region, we carried out size-based separation of PCR products, which was produced by using primers designed to minimize the stutter production. Blood and hair shaft samples were collected from 25 individuals. The result showed significant qualitative/quantitative peak pattern variations among blood and hair shaft mtDNA profiles. Based on the results of this study, an exclusion depended solely on differences in length of the major C-tract variant could thus be an erroneous interpretation. Therefore, differences in the number of cytosine or qualitative/quantitative peak pattern variations in the C-tract of the mtDNA HV2 region cannot be used alone to support an interpretation of exclusion.
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KEYWORD
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mtDNA, Length heteroplasmy, HV2, Hair shaft, Intra-individual difference
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